Evaluation of the potential of Raman spectroscopy for prediction of chemotherapeutic response to cisplatin in lung cancer cells
Personnel: H Nawaz, A Meade, F. Bonnier, HJ Byrne, FM Lyng
Funding source: HEA PRTLI Cycle 4 National Biophotonics and Imaging Platform
The in vitro study of the interaction of anticancer drugs with mammalian cells is important to elucidate the mechanisms of action of the drug on its biological targets. In this context, Raman spectroscopy is a potential candidate for high throughput, noninvasive analysis.
The interaction of cis-Diamminedichloroplatinum (II) (Cisplatin) with a human lung adenocarcinoma cell line (A549) was investigated using Raman microspectroscopy. The results were correlated with parallel measurements from the MTT cytotoxicity assay. To further confirm the spectral results, Raman spectra were also acquired from DNA extracted from A549 cells exposed to cisplatin. Partial least squares (PLS) multivariate regression and PLS Jack-knifing were employed to highlight spectral regions which varied in a statistically significant manner with exposure to cisplatin and with the resultant changes in cellular physiology measured by the MTT assay.
The results demonstrate the potential of the cellular Raman spectrum to non-invasively examine spectral changes that have their origin either in the biochemical interaction of external agents with the cell or its physiological response, allowing the prediction of the cellular response and the identification of the origin of the chemotherapeutic response at a molecular level in the cell. Raman spectroscopy, therefore, shows great potential for prediction of chemotherapeutic response in tumour tissue.
