• TFBT3003
  • Biotechnology

  • Credits (ECTS): 5
  • Food Science and Environmental Health

Modules are delivered
as part of a programme.
To apply for the
programme,
see the DIT website

Overview

Module Description

Introduction to fundamental principles of biotechnology and recombinant DNA techniques, including background, commonly used techniques and applications in the Pharmaceutical and Food sectors

Module Aims

The module aims to equip the student with practical and cognitive skills to perform basic molecular biology techniques.

Indicative Syllabus

Structure & function of nucleic acids, processes of genome replication and gene expression, mutations
Gene regulation in Prokaryotes & Eukaryotes, Lac operon, RNA splicing
Gene Cloning, Restriction enzymes, Transformation methods,
Expression systems used in industry
Selection/screening methods,
Monoclonal antibodies, Diagnostic test kits
Polymerase Chain Reaction (PCR) and applications
Protein engineering, methods and applications in the food & pharmaceutical sector
Laboratory Practicals Restriction analysis of DNA & Gel electrophoresis
DNA Transformation;
Plasmid isolation and analysis
PCR and Analysis of PCR products.

Total Contact Teaching Hours:48

Please note that the catalogue is provided as a guide to modules in DIT. Not all modules listed will necessarily be offered every year and new modules may also be added. Information subject to change. For detail on specific programmes/modules please contact the relevant School directly.