Beckman P/ACE MDQ Capillary Electrophoresis System

CES Overview:

Capillary electrophoresis (CE), also known as capillary zone electrophoresis (CZE), can be used to separate ionic species by their charge and frictional forces. In traditional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an electric field. Introduced in the 1960s, the technique of capillary electrophoresis (CE) was designed to separate species based on their size to charge ratio in the interior of a small capillary filled with an electrolyte.

Capillary Electrophoresis (CE) technology is employed in a series of related separation techniques that use narrow-bore fused-silica capillaries to separate a complex array of large and small molecules. Depending on the types of capillary and buffers used, CE can be segmented into:

-Capillary Zone Electrophoresis (CZE)
-Capillary Gel Electrophoresis (CGE)
-Capillary Isoelectric Focusing (CIEF)
-Isotachophoresis (ITP)
-Electrokinetic Chromatography (EKC)
-Micellar Electrokinetic Capillary Chromatography (MECC)
-Capillary Electrochromatography (CEC)
-Non Aqueous Capillary Electrophoresis (NACE)

CES1 Technical Specifications:

Modes of Operation Constant/Gradient Voltage†

Constant/Gradient Current†

Constant/Gradient Power†

Variable pressure

Sample Introduction Pressure, Vacuum, Electrokinetic

Voltage Range 1 - 30 kV (1 - 10 kV for injections),

Programmable in 0.1 kV increments

Reversible voltage programmed through user interface.

Current Range 3 - 300µ Amps

Pressure Range -5 to +100 psig (Rinse/Separations); -5 to +25 psig (Sample Introduction)

Sample Temperature 5° C to 60° C

Sample temperature environment is independent of the ambient buffer environment.

System Capacity:

Sample Tray 2 x 96-well plates

2 x 48 2 mL vials, 2 x 48 0.5 mL vials, 2 x 48 PCR vials

Buffer Tray 2 x 36 2 mL vials, 4 x 25 mL trays

Capillary Cartridge Recirculating liquid coolant 15° to 60° C

Wavelength Range Diode Array 190 - 600 nm

UV/Vis 200, 214, 254, 280 nm standard filter

190 - 600 nm (with custom filter options)

Detectors Diode Array

UV/Vis

Laser-Induced Fluorescence

Scan Collection Diode Array 0.5 - 32 Hz (user selected)

Data Rate Collection 0.5 to 32 Hz

Electrical Voltage 90 - 240 V 50/60 Hz